Profit margin is calculated on the selling price: Margin = Profit ÷ Selling Price. Markup is calculated on the cost price: Markup = Profit ÷ Cost Price. A 25% margin = 33.3% markup. A 20% markup = 16.7% margin. Always specify which you mean when discussing pricing.
For B2B transactions, GST is neutral to margin because both you and your buyer are registered — you collect GST and remit it, your buyer reclaims it as ITC. For B2C, if you are absorbing GST rather than adding it on top, your effective margin reduces. Always price your B2C goods with GST built into the customer-facing price.
If ITC is blocked on your purchases (e.g., you buy a car for business and GST is blocked), include the full cost price INCLUDING GST in your cost base. The blocked GST becomes part of your cost, not a recoverable credit.